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Image Search Results
Journal: Connective tissue research
Article Title: GREM1 inhibits osteogenic differentiation, senescence and BMP transcription of adipose-derived stem cells.
doi: 10.1080/03008207.2020.1736054
Figure Lengend Snippet: Figure 3. GREM1 overexpression inhibited ADSC senescence. (a) SA-β-gal staining showed fewer positive cells in GREM1 over- expressing ADSCs compared to vector control. Scale bar: 100 μm. (b) A quantitative analysis of SA-β-gal positive cells. (c) The relative telomerase activity was measured by the telomerase ELISA kit. (d, e) Real-time RT-PCR results showed that GREM1 overexpression decreased the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.
Article Snippet: We measured the
Techniques: Over Expression, Staining, Expressing, Plasmid Preparation, Control, Activity Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR
Journal: Connective tissue research
Article Title: GREM1 inhibits osteogenic differentiation, senescence and BMP transcription of adipose-derived stem cells.
doi: 10.1080/03008207.2020.1736054
Figure Lengend Snippet: Figure 4. GREM1 knockdown accelerated ADSC senescence. (a) SA-β-gal staining showed an increased number of positive cells in the ADSC-GREM1sh group compared to the control group. Scale bar: 100 μm. (b) The quantitative analysis of SA-β-gal positive cells. (c) Telomerase activity assay results showed that GREM1 knockdown significantly decreased ADSCs telomerase activity. (d, e) Real- time RT-PCR results showed that GREM1 knockdown upregulated the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.
Article Snippet: We measured the
Techniques: Knockdown, Staining, Control, Telomerase Activity Assay, Activity Assay, Quantitative RT-PCR